An Excel file is simply a data file (though, being a Microsoft file format, it contains executable data, which is antithetical to any sort of security whatsoever). Since an Excel file is merely a data file, an application can do whatever it pleases such as reading and writing to any "cell" on any "sheet" in the file. This is a function of the application generating or working with the file. For example, there are (at least) two libraries for Python (that I know of and use) -- openpyxl and xlrd -- that will let a python application have its way (as it were) with Excel files. Given that an Excel file is nothing more than a data file, there are most likely libraries written in other languages to permit one to have their way with Excel files. If you mean can that piece of software known as "Microsoft Excel" do this, that is something that you will have to take up with Microsoft. SQLite3 (the library) does not work with Excel files. It works with SQLite3 database files and provides an API so applications can use the database files. There are "extensions" which can import and export CSV files, but none that work with Excel files directly. One such Application Program is the SQLite3 shell. It is designed for convenient and generic command line access to SQLite3 database files. It has some rudimentary I/O capabilities for dealing with the console and importing and exporting data in various formats such as CSV. It does not work with Excel directly. So, your question is somewhat unclear because yes, an application running on a general purpose computer can do whatever your little heart desires limited only by your skill in defining and writing the application. So the answer is yes. An application can be written that does what you want. Has it been done? Yes it has (I wrote one, for example, but you cannot have that one). Can an application that is freely available to you that you did not write do that? No idea. Google is your best buddy in that case. Can SQLite3 do that? No. The purpose of SQLite3 is to work with SQLite3 database files. You might write an application that does that that also uses the SQLite3 library to interface with SQLite3 database files, however, that is the business of the application and not of SQLite3.